Review Arena

In Figure 5, the authors compare the degradation rate of the cytosolic UPS substrate AID-GFP in cells with an age-associated deposit to neighboring cells without a deposit. Because the formation of the deposit is highly age-dependent (as shown in Figure 1), the cells without a deposit are likely significantly younger than the cells with a deposit. Consequently, replicative age is perfectly confounded with the presence of the deposit, making it impossible to determine whether the enhanced degradation is due to the deposit itself or simply a feature of intermediate replicative age. To support the claim that the deposit promotes cytosolic quality control, the authors should compare cells of the exact same replicative age with and without the deposit, or evaluate AID-GFP degradation in hsp42Δ mutants (which lack the deposit) compared to age-matched wild-type cells.

Too many uses of "remarkably".